Anti Diabetic and Anti Cancer Effects of Silver Nanoparticles Synthesised from Botryodiplodia theobromae- An Endophytic Fungi isolated from Euphorbia hirta – A Weed

摘要

Weeds are a plant that is out of place and not intentionally sown.A plant that grows where it is not wanted or welcomed. Endophytes are ubiquitous and have been found in all the species of plants studied to date; however, most of these endophyte/plant relationships are not well understood. In order to extend the observations on the potential inhibition of human colon adenocarcinoma cells, the present study evaluated the effect of silver nanoparticles synthesised by Botryodiplodia on HT-29 cells in vitro, and investigated the possible underlying molecular mechanism. The fungal cell filtrate after addition of aqueous AgNO3 (1 mM) was subjected to optical measurements by UV-Vis spectrophotometer; this analysis showed an absorbance of peak at 420 nm. The FT-IR measurements of the cell filtrates, confirmed the existence of free OH and NH groups, aromatic CH stretching, C - C stretching and the existence of –C ≡ C – H: C – H bend. The size of the nanoparticle produced was ranging from 53nm – 79 nm and 48 nm - 68 nm from the cell filtrate and fungal mat respectively. The XRD pattern of the test sample exhibited peaks 38?, 44°, 64 ° and 77° corresponding to 111, 200, 220 and 311 planes were recorded. The XRD result discloses the formation of pure crystalline. The silver nanoparticles synthesised from fungi Botryodiplodia theobromae shows the highest percentage of inhibition for α-amylase (94%). The MTT assay clearly evidenced that on using different concentrations the cytotoxicity was more when 100 μl of the sample was used.