Study of Mitochondrial DNA Copy Number Variation in Peripheral Blood of Type 2 Diabetes Patients: A Pilot Study

摘要

Variations in content of mitochondrial DNA are known to be associated with different metabolic conditions such as diabetes, cancer, obesity and cardiac diseases. But there are few reports in case of Type 2 Diabetes patients and there is need of comprehensive studies which can correlate the variation in mitochondrial DNA content with T2DM. Our proposed study is an attempt to understand the correlation of mt DNA content with different clinical features of T2DM patients. Our current study aims to investigate the copy number variation of mt DNA in total 40 well characterized T2DM patients. Ten healthy age matched individuals were also involved in the study as a control. For the study of the relative mt DNA copy number measurements, Real time PCR using SYBR green method was performed and statistical analysis was done using SPSS 21.0.Following the real time PCR and ??Ct method of analysis, it was observed that in 80% of the studied patients, the content of mitochondrial DNA was found to be increased in comparison to control. The statistical studies further suggested that there was a slight positive correlation between mtDNA content and HbA1C of these patients. As increased mitochondrial content is proposed to be a marker for disease phenotype and our study strengthen this hypothesis.