Hepatoprotective Effect and In Vivo Antioxidant Activity of Stem Barks of Hibiscus vitifolius Linn against Paracetamol Induced Hepatotoxicity in Rats”

摘要

The objective of this study was to evaluate the hepatoprotective activity and in vivo antioxidant activity of Hibiscus vitifolius Linn stem barks against paracetamol induced hepatotoxic model. Dried stem barks were coarsely powdered and extracted with petroleum ether, chloroform, ethyl acetate and methanol by successive extraction in a soxhlet apparatus for 72 h. Preliminary phytochemical test of ethyl acetate extracts of Hibiscus vitifolius (EAHV) shows the presence of carbohydrates, phenolic compounds and flavonoids. Ethyl acetate extracts was found to be safe upto a dose of 2000mg/kg. The EAHV showed a significant dose dependent (50 mg/kg & 100 mg/kg p.o) were evaluated for their possible hepatoprotecive and in vivo antioxidant potential. All the parameters were compared with control. There was a significant (p<0.01) reduction in the level of AST, ALT, ALP, total and direct bilirubin, where as an increase was found in total protein. In the tissue anti-oxidant studies, we found a significance increase in the levels of catalase (CAT), superoxide dismutase (SOD) and GPx where as there was marked reduction in the level of malondialdehyde, thiobarbituric acid reactive substance. Histology of liver sections of the animals treated with the ethyl acetate extracts showed significant reduction of necrosis and fatty formation when compared with control specimen. The effect of EAHV was comparable to that of standard drug silymarin.