FORMULATION, ASSESSMENT AND COMPATIBILITY ANALYSIS OF DIFFERENT POLYMERS LOADED MICROSPHERES BY NON AQUEOUS SOLVENT EVAPORATION TECHNIQUE: IN VITRO-IN VIVO STUDY OF GLIBENCLAMIDE AS A MODEL DRUG

摘要

Glibenclamide is an oral anti-hyperglycemic agent designed intended for the management of non-insulin-dependent diabetes mellitus (NIDDM). In certain conditions conventional drug release pattern is not suitable similar to Diabetes mellitus, cardiovascular diseases and many more diseases, this present study has taken a challenge to formulate controlled release microspheres by using different polymers. An effort has been given to prepare controlled release microspheres along with Ethyl cellulose, Eudragit RS/RL100 and Methocel K15, 100M by using non-aqueous emulsion solvent evaporation method. UV-Spectrophotometric was applied to assay the drug content and in vitro dissolution studies according to USP paddle method and was carried out in Phosphate Buffer (pH 7.4) for 8 hours. The in-vitro release kinetics was studied in different mathematical release models. The best data fitted with the highest correlation coefficient (R2) for microspheres was obtained for Korsmeyer release model. The maximum and minimum release of drug was observed 90.99% and 71.98%. Percent of Actual drug entrapment varied from 7.89% to 15.36% and percent of Drug entrapment efficacy varied from 69.23% to 98.21%. The SEM, FTIR and DSC studies used to of confirmed good spheres and smooth surface as well as interaction along with drug and polymer. Different biochemical tests have done on albino rats which showed good results and it also proved that formulations have given sustained release effects. In-vitro-in vivo studies showed that Glibenclamide microspheres might be a good candidate as sustained drug delivery system for treating type II diabetic