RENO-HEPATOPROTECTIVE EFFECTS OF MURRAYA KOENIGII LEAVES CHLOROFORM EXTRACT (MKCE) AGAINST LEAD-INDUCED OXIDATIVE STRESS IN MICE

摘要

Lead induced oxidative stress implies morphological dysfunctions and physiological deformation in the liver. The aim of present study was to establish the protective effect of chloroform extract of Murraya koenigii leaves against the lethal effects of lead on the antioxidant status of mice. Murraya koenigii Chloroform Extract (MKCE) was prepared by maceration. Male Swiss albino mice were divided into three groups of six each. Group I (Control group) served as normal diet and water ad libitum, Group II (lead treated group) received an intraperitoneal injection of lead acetate (15mg/kg) daily once whereas Group III (MKCE with lead-treated group) received MKCE (50 mg/kg) orally along with a single of injection of lead acetate intraperitoneally (15mg/kg) daily once. Experimental study was continued for the consecutive seven days. On the 8th day, liver and kidneys were desiccated; homogenized in normal saline to collect supernatant into vials for estimating serum malondialdehyde, antioxidant enzymes like superoxide dismutase, catalase, reduced glutathione, glutathione peroxidase, glutathione-s-transferase and ferric reducing ability of plasma levels. It was observed that significant alteration in levels of antioxidant parameters in lead-intoxicated mice. Antioxidant parameters were significantly restored in group III. Results in the study revealed that MKCE has antioxidative effects against lead induced oxidative stress in mice.