Integrated Waste-Metal-Bioenergy: An Operative Approach for Water Remediation Waste to Bio-energy

摘要

The objective of this research was to develop simple, rapid, precise, accurate and economical stability-indicating reversed phase (RP) HPLC assay method and validated for simultaneous estimation of Lamivudine, Abacavir and Dolutegravir in bulk laboratory synthetic mixture and their combined dosage form. The method has shown adequate separation of Lamivudine (LAM), abacavir (ABA) and Dolutegravir (DOL) from their degradation products. Separation was achieved on Luna Phenyl Hexyl, (250 mm x 4.6mm, 5μm) column, using mobile phase acetonitrile and 0.1 M Ortho phosphoric acid buffer using a gradient program with a flow rate of 1ml/min, throughout the gradient program with a detection wavelength of 258 nm for all the compounds with injection volume of 20μl. The retention times for Lamivudine, Abacavir and Dolutegravir were found to be 3.3, 4.5 and 6.3 min respectively. The linearity range of LAM, ABA and DOL were 3-45 μg/ml, 6-90 μg/ml and 0.5-7.5 μg/ml respectively with a correlation coefficient of 0.999. The method was validated as for ICH Q2 R1 guidelines. The method was validated for selectivity, linearity, accuracy, robustness, precision and specificity and the obtained results are within acceptance range. The results were indicating the method was selective in analysis of all Lamivudine, Abacavir and Dolutegravir in the presence of degradation products formed under various stress conditions.