“In Vitro Callus Induction of Ceropegia bulbosa and Ceropegia attenuata”–An Endangered Tuberous Plants of Rajasthan

摘要

The genus ceropegia belongs to the Asclepiadoideae (milkweed) sub-family within the family Apocynaceae. Ceropegia species is a medicinal herb, this is useful in curing many diseases like kidney stone and deafness which in present time cured surgically but this plant product remove it naturally so this plant has great medical uses. Ethno botanical study showed that the plant has good medicinal value for tribe of Rajasthan. The effect of various phytohormones on in-vitro callus induction and shoot regeneration in Ceropegia species from leaf explant was evaluated. The sterilized explants were inoculated on ms medium supplemented with different concentrations of auxin alone or in combination with cytokinins. Best result was observed on MS medium fortified with NAA (1.5 mg/l). Callus generation capacity, length and morphology were observed. In vitro generated callus was used further for large scale production of plant and biochemical study.