RNA: DNA Ratio and Growth Performance of Rohu Labeo rohita (Hamilton) Fed Varied Proportion of Protein Diet during Intensive Aquaculture

摘要

An experiment was conducted at Corona of Agriculture (COA)”, Gunjanagar-3, Chitwan, Nepal to complete a project granted by University Grant Commission (UGC), Second Higher Education Project (SHEP) in 2013. Four (average 380 m2) ponds (W, X, Y and Z) with four different diets as D1 (20%), D2 (30%), D3 (40%) and D4 (50%) protein contents were used to conduct the experiment properly to determine the varied proportion of dietary protein on the growth performance of major carp rohu Labeo Rohita (H) in relation with RNA: DNA ratio. After 12th weeks of culture, average length, average weight and specific growth rate of fish were found significantly (P<0.05) higher in the carp with D3 and D4 diet fed fish. Similar results were observed in average total protein, albumin and globulin contents. RNA content increased rapidly with age. The average RNA content increased highest in D2 and D3 diet fed fish while, DNA content were highest in D3 diet fed fish. RNA: DNA ratio was recorded highest in D2 and D3 diet fed fish. RNA: DNA ratio, an indicator of protein synthesis and have been used to accurately estimate the growth rate and feeding condition of fish hence, as the dose of protein increased RNA and DNA contents also increased with age of carp cultured during experiment. Thus, it was clear from this study that the incorporation of protein in diet enhances the growth of fish regardless of species weight groups and the doses, as the average weight of fish was significantly lower in control diet fed fish as compared to the treated one.