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首页> 外文期刊>International journal of oncology >Molecular detection of circulating cancer cells in the peripheral blood of patients with colorectal cancer by using membrane array with a multiple mRNA marker panel
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Molecular detection of circulating cancer cells in the peripheral blood of patients with colorectal cancer by using membrane array with a multiple mRNA marker panel

机译：带有多个mRNA标记物的膜阵列分子检测大肠癌患者外周血中循环癌细胞的分子

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The objective of this study was mainly to evaluate the simultaneous detection of expression levels of a multiple mRNA marker panel in the peripheral blood of colorectal cancer (CRC) patients for use in complementary CRC diagnosis. Twenty-seven tumor tissue specimens and 80 peripheral blood specimens were collected from CRC patients. Firstly, the levels of multiple molecular markers in the tumor tissue and blood specimens were evaluated by using real-time quantitative PCR (RT-QPCR) and membrane array. The result of linear regression showed a high degree of correlation (r=0.954, P<0.0001) between the data of these two methods. CK-19 was the marker with the highest detection rate (87.5%) in the peripheral blood, followed by CEA (82.6%), REG4 (80.8%), and then uPA (80.0%) and TLAM1 (80.0%). The levels of the six markers in the peripheral blood were extensively explored. In the 80 patients, the frequency of CK-19, CK-20, CEA, REG4, uPA, and TIAM1 mRNA overexpression was 82.5% (66/80), 78.8% (63/80), 82.5% (66/80), 80.0% (64/80), 78.8% (63/80), and 80.0% (64/80), respectively. Then, a panel combining these 6 mRNA markers was evaluated for its utility in the clinical diagnosis of CRC. The sensitivity, specificity, and accuracy of membrane array-based diagnostic method were 88.8%, 87.8%, and 88.2%, respectively; much higher than those of examinations with single markers. Finally, lymph node metastasis (P=0.024) and TNM stage (P=0.009) were found to be significantly correlated with overexpression of the multiple mRNA marker panel. The detection rates of stage-I and -II CRC by using the multi-marker membrane array were 54.5% (6/11) and 92.0% (23/25), respectively. In conclusion, the results of the present study have shown that this innovative membrane array technique with a multiple mRNA marker panel can significantly improve the diagnosis rate of early colorectal cancer.
机译：这项研究的主要目的是评估同时检测大肠癌（CRC）患者外周血中多个mRNA标记物组的表达水平，以用于辅助CRC诊断。从CRC患者中收集了27份肿瘤组织标本和80份外周血标本。首先，通过实时定量PCR（RT-QPCR）和膜阵列技术评估了肿瘤组织和血液样本中多种分子标记的水平。线性回归的结果表明这两种方法的数据之间具有高度相关性（r = 0.954，P <0.0001）。 CK-19是外周血中检出率最高的标志物（87.5％），其次是CEA（82.6％），REG4（80.8％），然后是uPA（80.0％）和TLAM1（80.0％）。广泛探索了外周血中的六种标志物的水平。在80例患者中，CK-19，CK-20，CEA，REG4，uPA和TIAM1 mRNA过表达的频率分别为82.5％（66/80），78.8％（63/80），82.5％（66/80） ，80.0％（64/80），78.8％（63/80）和80.0％（64/80）。然后，评估组合这6种mRNA标记的面板在CRC临床诊断中的效用。基于膜阵列的诊断方法的灵敏度，特异性和准确性分别为88.8％，87.8％和88.2％。比单笔考试要高得多。最后，发现淋巴结转移（P = 0.024）和TNM分期（P = 0.009）与多种mRNA标记物组的过表达显着相关。使用多标记膜阵列的I期和-II期CRC检出率分别为54.5％（6/11）和92.0％（23/25）。总之，本研究的结果表明，这种具有多个mRNA标记面板的创新性膜阵列技术可以显着提高早期大肠癌的诊断率。

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