Hydrated Fullerene C60 Decreases Oxidative Damage of Human Bronchial Epithelium Cells DNA

摘要

Background: Hydrated Fullerene C60 is a 60 carbon atoms symmetrical molecule that can be foundin nature. Being transferred into aqueous solution, a hydrophobic C60 establishes HydratedFullerene C60 (HyFnC60) water solutions that possess oxidation balancing properties in a wide rangeof concentrations (2.5×10-6 – 2.5×10-19 М). Nano-particles of silica (SiO). Nano-particles of silica (SiO2) can initiate reactive oxygenspecies (ROS) production in aqueous solutions. As silica is a common pollutant and a component ofmanufactured goods, one of the most probable ways of its penetration into human body isinhalation followed by an increased risk of chronic inflammation and cell degeneration due to ROSproduction inside the cells and in the extracellular space.Aims: In this study we focused on the possibility of DNA protection of human bronchial epithelialcells (HBEpCs) of oxidative damage by silica nanoparticles with HyFnC60 in a wide range ofconcentrations.Methodology: Cultivated cells were treated with 50 mg/ml nano-sized SiO2. HBEpCs DNA strandbreaks were accessed by comet assay. Intracellular ROS production was measured by DCFH–DAfluorescent spectrophotometry. HyFnC60 were added to the cell culture medium before treatmentwith silica. The calculated concentrations of HyFnC60 in the medium were 1×10-7 М). Nano-particles of silica (SiO 1×10-11 М). Nano-particles of silica (SiO,1×10-14 М). Nano-particles of silica (SiO and 1×10-17 М). Nano-particles of silica (SiO. The solutions of HyFnC60 were prepared by 10- and 100-fold serialdilutions of HyFnC60 stock solution in pure water. Results were evaluated statistically by Student`st-test and variance analysis by Statistica? software.Results and discussion: We have found that in case of pre-treatment of cells with 1×10-7 М). Nano-particles of silica (SiO and1×10-17 М). Nano-particles of silica (SiO HyFnC60 prior addition of silica DNA damage was decreased. According to DCFH–DAfluorescent microscopy a significant decrease of ROS was observed in case of addition of HyFnC60 tothe concentration of 1×10-7 М). Nano-particles of silica (SiO only. In case of simultaneous treatment of cells with silica andHyFnC60 cell damage was decreased at HyFnC60 concentration of 1×10-17 М). Nano-particles of silica (SiO. This data proves thatDNA was damaged by ROS induced by silica nanoparticles. Moreover, the data confirm thatHyFnC60 works as regulator of redox and free radical processes at low concentrations.