Molecular Analysis of Bulgarian Common Bean Genotypes and their Characterization after Growing under Rainfed and Irrigated Conditions

摘要

Experiments were conducted in the field of Agricultural University in Plovdiv, Bulgaria. A standard method for cultivation in 5 replicates was applied. Biometric evaluation of common beans (Phaseolus vulgaris L.) - 10 mutant lines and 10 varieties, grown under rainfed and irrigated conditions was conducted. Main traits, associated with productivity in common beans: plant height, mass of plants with pods, number of branches, height of betting on the first pod, number of fruit branches, number of pods per plant, weight of pods with seeds, number of seeds per plant, weight of seeds and average length per 10 pods, were haracterized. Mutant line D2-0.0125 M EMS (6) has the best manifestation of the studied traits among other mutant lines and it may be included in breeding schemes for evaluation as a new cultivar. BAT 477 (20) differs significantly by its traits from other genotypes, irrespectively of the cultivation mode. RAPD and ISSR analyses were done to the studied genotypes. On the basis of molecular characterization clear allocation of genotypes was found on dendrogrames bilt by NTSYS programe. It was demonstrated that the studied Bulgarian varieties are promising germoplasme for their introduction in hybridisation breeding schemes, as well as in application of mutagenesis and biotechnological aproaches.