Electrochemical active biofilms (EABs) have attracted considerable attractions in bioelectrochemicalsystems (BESs) field for pollutants degradation from waste water. However, few studies have lookedat EABs formation influenced by anode and cathode conditions. Therefore, EABs feature wasinvestigated under different electrode conditions using a single-chamber BES. After EABs formation,the BES was effective in decolorizing the targeted pollutant azo dye, comparing to abio-electrodeBESs. By analyzing the EABs microbial community compositions, anode EAB was dominated by amodel electro-active Geobacter (29%), whereas the cathode EAB microbial structure was relativelycloser to the inoculum with low abundance Geobacter (2.5%). This study suggested that changing theinoculum composition would be conducive to improving the cathode EAB capability for recalcitrantpollutant degradation in actual engineering applications.
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