Highly Sensitive Pencil-Based Renewable Biosensor for Hydrogen Peroxide Detection With a Novel Bionanomultilayer

摘要

A renewable graphite pencil electrode was demonstrated to be used as excellent working electrode foramperometric detection based on a novel bionanomultilayer using layer-by-layer assembly of goldnanoparticles and horseradish peroxidase. Parameters affecting the performance of the biosensor wereoptimized to obtain the maximal sensitivity. The resulting bionanomultilayer provided a linear range-1 -2 between 0.01 and 1.5 mM with the sensitivity of 149 A mM cm and a detection limit of 0.002mM. The biosensor was successfully applied to detect the amount of hydrogen peroxide in hair dyeand disinfector samples. The results were well agreed to classical titration method. The recovery ofhydrogen peroxide detection ranged from 80 to 104%.