Construction and Performance Characterization of Ion Selective Electrodes for Potentiometric Determination of Ranitidine Hydrochloride in Pharmaceutical Preparations and Biological Fluids

摘要

The construction and performance characteristics of ranitidine hydrochloride (RNH) selectiveelectrodes are described. Two types of electrodes: carbon paste and screen printed electrodes wereconstructed based on the ion-pair formation during potentiometric titration of ranitidine hydrochloridewith sodium tetraphenylborate. The electrodes were fully characterized in terms of plasticizer type,response time, lifetime, soaking, titrant, pH and temperature. The CPE and SPE electrodes exhibited Nernstian response for RNH in the concentration range from 1.010 to 1.010 and 1.010 to -1 -1 1.010 mol L with a slope of 55.741 and 58.891.2 mV decade , respectively. The detection limit-7 -7 -7 -7 -1 and quantification are found to be 0.0710 and 0.2310 and 0.0910 and 0.3210 mol L forCPE and SPE, respectively. The standard electrode potentials, E, were determined at 10, 25, 30, 40and 50 C and used to calculate the isothermal temperature coefficient (dE/dT) of the two electrodes.Temperatures higher than 50 C seriously affect the electrodes performance. The direct potentiometricdetermination of RNH using the proposed sensors gave average recoveries % of 98.000.40- 98.570.37 and 98.680.42-99.330.31 for CPE and SPE sensors, respectively. The electrodes wereapplied for potentiometric determination of RNH in pure state and pharmaceutical preparation underbatch conditions, urine and serum. Interference studies from a large number of inorganic cations,sugars, glycine and drug excipients are reported. The electrodes gave average selective precision andwere usable within the pH range 3-5 and 3-6 for CPE and SPE, respectively. The developed methodwas found to be simple, accurate and precise when compared with the reported method.