DNA-based Biosensor for Detection of Ganoderma boninense, an Oil Palm Pathogen Utilizing Newly Synthesized Ruthenium Complex [Ru(phen)₂(qtpy)]2+ Based on a PEDOT-PSS/Ag Nanoparticles Modified Electrode

摘要

An electrochemical DNA biosensor has been developed for detection of ganoderma boninense, an oil2+ palm pathogen utilizing newly synthesized ruthenium [Ru(phen)2(qtpy)] complex as hybridizationindicator. The sensor incorporated the use of a gold electrode (AuE), modified with a conductingnanocomposite of poly(3,4-ethylene- dioxythiophen) - poly(styrenesulfonate) (PEDOT-PSS) and silvernanoparticles (AgNPs). A specific sequence of a ganoderma boninense DNA probe has beenimmobilized on the modified electrode and the hybridization event was monitored via intercalation ofthe ruthenium complex to the hybridized DNA. Effect of hybridization temperature and time waso evaluated and found to be optimal at 45 C in 25 minutes for the hybridization. Detection of target-15 -9 DNA ranged from 1.0 x 10 M to 1.0 x 10 M was performed, and a correlation relationship of-16 0.9756 and detection limit of 5 x 10 M were obtained. The newly synthesized ruthenium complexwas able to be used is a novel redox marker and can be adopted for routine detection of DNA.