Physicochemical studies of closed loop insulin delivery system based on intelligent carboxymethyl cellulose hydrogel

摘要

Diabetes mellitus type 1 (T1DM) is a form of diabetes mellitus that results from the autoimmune destruction of? the insulin producing β-cells in the pancreas. This study aims to establish the safety and efficacy of a hybrid closed loop . Physicochemical studies, we investigated for a closed loop insulin delivery system based on intelligent carboxymethyl cellulose (CMC) hydrogel. The hydrogel was synthesized by free radical polymerization of acrylamide (AAm) in the presence of CMC in atmospheric condition. Immobilization and loading of glucose oxidase (GOx) and insulin was done by the swelling diffusion method. GOx plays an important role to make the synthesized hydrogel responsive to blood glucose level as a model of an artificial pancreas. The loaded hydrogels were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), high- performance liquid chromatography (HPLC) and thermo gravimetric analysis (TGA). Thermodynamic parameters such as Δμ, ΔH, ΔS, E_(a), and coefficient diffusion were also calculated. The results indicate that hydrophobic forces play a major role in drug-hydrogel interactions.