Serological and biological characterization of Zucchini yellow mosaic virus (ZYMV) infecting cucumber in Pothowar, Pakistan

摘要

A survey was conducted during 2013-14 in open growing cucumber fields of Pothowar region in order to explore the prevalence of Zucchini yellow mosaic virus (ZYMV) through biological and serological assay. Leaves and fruits showing symptoms like mottling, mosaic, shoe string , knobby appearance, yellowing were collected randomly. Collected samples were tested byDAS-ELISA (Double Antibody Sandwich- Enzyme Linked Immunosorbant Assay) by using ZYMV virus specific antisera and through bioassay. Highest disease incidence was noted in Rawalpindi i.e. 59% followed by 53% in Islamabad, 33% in Attock as well as in Jhelum and 28% were recorded in chakwal. During this research study it is also monitored that aphid vector Myzus persicae , and Aphis gossypii transmit virus in non-persistent manner but the rate of transmission of Myzus persicae was little higher than Aphis gossypii.