Multiple Shoot Regeneration of Roselle ( Hibiscus sabdariffa L.) from a Shoot Apex Culture System

摘要

The present study reports a protocol for the efficient in vitro regeneration of Hibiscus sabdariffa using the shoot apex as explant. Shoot tips were cut 10 days after germination on MS medium complemented with 4.43 ?? M of 6-benzyladenine (BA). Different concentrations of BA (8.87-35.48 ??M), thidiazuron (0.91-18.16 ??M) and m -topolin (8.28-33.12 ??M) in the MS medium were assessed for shoot production. A considerable variability in regeneration efficiency among the growth regulators used was observed as shoots developed over the apical meristem in a rosette disposition. The highest number of shoots (33) was obtained with the 17.74 ??M BA treatment, compared to the 16 shoots obtained with 16.56 ??M m -topolin. The shoots obtained were placed in MS media without growth regulators in total darkness to induce stem elongation. Production of roots was abundant, obtaining 98.6% of regenerated plants with the BA and m -topolin treatments. The genetic variation analysis through RAPD showed a uniform polymorphic pattern to the reganerated plants indicating that they were genetically homogeneous. This research is the first report of the inductive effect that BA has on the production of multiple plants from a shoot apex of H. sabdariffa and the possible regeneration of plants without a rooting medium.