Automated Parasite's Detection in Microscopic Images of Stools Using Distance Regularized Level Set Evolution Initialized with Hough Transform

摘要

Background and purpose: The analysis of biomedical microscopic images is carried out manually in medical laboratories. The manual analysis of clinical images lets to both repetitive tasks and management of huge amounts of data. This is tedious and times consuming for laboratory technicians. Inevitably, it is also prone to human errors. Our objective in this work is to contribute to the automation of the analysis of microscopic images of stools using Distance Regularized Level Set Evolution automatically initialized by Hough transform. Method: We firstly converted the microscopic images to edge maps using canny algorithm. Next, we located the parasite through circular Hough transform and draw circles around them. Those circles stand as initial contours of DRLSE. The contours evolve until they fit the boundaries of the parasites. The final extraction is performed using a complementary method based on the signed distance character of the level set function. Results: The Distance Regularized Level Set Evolution has been automatically initialized. We applied our method to the detection of intestinal parasites in microscopic images. Experimental results show accurate, efficient and less time consuming of our scheme compared to others recently proposed in the literature. Conclusion: This is a notable contribution to the automation of stools examination in the medical laboratories. In forthcoming works, we plan to include this segmentation process in an expert system of parasitic diseases diagnosis.