Agrobacterium mediated production of transgenic tomato plants harbouring AP24 gene from tobacco

摘要

The tobacco (Nicotiana tabacum) antifungal AP24 protein identifies as osmotin encodes by its correspondinggene and contributes to pathogen resistance in plants .In the present study the AP24 gene was obtained fromtobacco genome by PCR amplification. Following of this successful amplification, transgenic tomato (Solanumlycopersicum) which constitutively expressing the AP24 gene was generated by Agrobacterium tumefaciensmediated transformation. In order to express AP24 protein in tomato, the gene was inserted into a plantexpression vector PBI121 and the recombinant pBI-AP24 plasmid was proliferated in E.Coli TG1. The newconstruct was used to transform the Strain Agrobacterium LBA4404 before plant transformation. Thentransgenic tomato plants were developed by introducing AP24 gene in the plant genome under the control ofCaMV35s promoter. In order to show the stability of AP24, transgenic T1 tomatoes analyzed by PCR and hightransformation proficiencies obtained. Releasing this one new transgenic variety may be a considerable progresstoward release varieties enables to show resistant antifungal characteristics.