Data Collection using Cryo-cooling Technique and Reducing the Damage by Freezing Samples to Collect High-intensity Protein Data using Cryocrystallography

摘要

It is noteworthy that we have solved a good no. of protein structure (at in-house facility comprising RU300 RotatingAnode Generator, mar345dtb Imaging Plate, mar300 scanner and low temperature device from oxford system) nearly to atomicresolution.The low resolution technique would be used to get an overview and identify interesting regions and then incorporating the highresolution technique to zoom in and look at details pertaining to different techniques. In this context there are limitations relatedto radiation damages caused by high intensity beam from the Generator. Reducing the damage by freezing samples would allowinvestigations of structure but the dynamic part is of course not accessible any more. We are working to reduce the damagescaused by high intensity beam by innovating different techniques in this direction.