CARRAGHEEN MOLECULAR MARKER DATABASE (CAMM-DB): A COMPREHENSIVE DATABASE FOR CARRAGHEEN ( CHONDRUS CRISPUS ) MOLECULAR MARKERS

摘要

Objective: CaMM-Db has been developed to manage the molecular marker information on Chondrus crispus (carragheen) and to make it accessible to the biological community. The database contains derived microsatellites and SNPs (Single Nucleotide Polymorphisms) from carragheen genomic sequences. The purpose for which carragheen is used, is also achieved by other Indian red seaweeds like, Gracilaria and Hypnea species. Except carragheen, the genome sequences of none of the red seaweeds are available in public domain. Thus, an insight into carragheen genome will help enable the biotechnologists to work on carragheen and the other red seaweeds. Methods: Keeping the above in view, CaMM-Db was developed using the carragheen genomic sequences from the databases of National Center for Biotechnology Information (NCBI) for microsatellite determination and SNP discovery. Till date, no SNPs for Chondrus crispus are submitted to NCBI, thus, here an attempt has been made to discover SNPs from carragheen genomic sequences. Results: This database provides information on different types motifs categorized based on different properties. The database is further integrated with Primer3 to facilitate the generation of suitable primers of interest for wet lab experimentation. Conclusion: As it is the first database on the molecular markers in carragheen genome, it can be used as a valuable resource for the scholars indulged in genetic research on carragheen and other Indian seaweeds.