Predominance of Spirastrella inconstans Protein over Enzymes of Marine Bacteria against MRSA

摘要

Fisherman community people will have significant value if they used powder form of marine organism for wound healing caused by MRSA. The bioactivity was more prevalent in sponge, Spirastrella inconstans compared to other marine organism. The protein was fractioned by ammonium sulphate precipitation method and purified using dialysis. About 1000 ?l of dialysate were loaded on Sephadex G-50 chromatographic column and purified 75 fractions at each percentage of saturation were collected. The fraction no. 3, 4 (40% saturation) and no. 4 (60% saturation) bears maximum protein content. Amount of protein content was estimated in UV-Vis Spectrophotometer, preliminary detection of compound was carried out by TLC. Two fractions were efficient and their R f values were found to be similar to that of glutamic acid. The molecular weight of protein was calculated as 3 000 Da by SDS-PAGE. Marine bacterial cultures were isolated and enzymes (amylase, DNase, esculinase, lipase, gelatinase and alginase) were produced and lipase bearing maximum activity was treated against MRSA. Compared to other marine organism, sponge protein obtained during our period of study was effective.