EVALUATION OF MITIGATING EFFECT OF PURIFIED SOLASODINE FROM SOLANUM MAURITIANUM AGAINST HYDROGEN PEROXIDE INDUCED OXIDATIVE DAMAGE IN HUMAN ERYTHROCYTES

摘要

Solanum mauritianum (Solanceae) is an exotic species from South America . Solanum species are known for alkaloid content which marks them medicinally important. Knowledge of the secondary metabolites of plant is desirable, not only for the discovery of therapeutic drugs but also it disclose new sources of economically important product. Herbal products have received considerable attention in recent years due to their diverse pharmacological properties, including antioxidant and antitumor potential. Solasodine, the nitrogen analogue of diosgenin, has been reported as potential steroidal precursor for the supplementary source of the commercial synthesis of diverse steroidal drugs. Free radicals are formed during the course of normal metabolic process in the biological system. They are highly reactive molecules due to the presence of unpaired electron. In this juncture, present study was aimed to isolate and purify the alkaloid solasodine from fruits and leaf and to evaluate its antioxidant activity via the assays such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, nitric oxide, hydrogen peroxide, superoxide anion scavenging activities etc. The alkaloid solasodine was isolated from leaves and fruits of the species using non polar to polar solvents and subsequently purified using column chromatography. Remarkable antioxidant activities are shown by the alkaloid solasodine. IC 50 values were comparable with synthetic antioxidants such as ascorbate, rutin. Moreover, the solasodine exhibited concentration dependent inhibitory activity against the free radicals. Significant scavenging property was noticed especially with DPPH radicals. A ntihaemolytic activity of the solasodine was also confirmed from this study. Thus, it can be suggested that solasodine show more protective effects through antioxidant potential against H 2 O 2 induced oxidative damage on erythrocytes. So, solasodine can effectively be used as natural antioxidant for the treatment and prevention of lipid peroxidation related disorders.