Correlation of Chemiluminescence and Electrochemiluminescence Methods for Determination of Serum Free Triiodothyronine Level in Cow

摘要

The aim of this study was to determine serum free-triiodothyronine (fT₃) level and its correlation between the CLIA and ECLIA methods in cow. Blood samples were collected from the jugular vein of 25 clinically healthy animals aged from 2 to 4 years. This study was performed in August. The separated sera were analyzed to measurement of fT₃ concentration using the CLIA and ECLIA methods. Our results indicate that there was no a significant difference between two method concerning the fT₃ concentration in cow, but its mean was higher in CLIA method. The regression analysis revealed a significant correlation between two methods: (p = 0.022, r = 0.460 for fT₃). It was concluded that the CLIA and ECLIA methods can be used as an alternative to the previous methods for assaying the fT₃ concentration in veterinary diagnostic laboratories and exhibited an acceptable of sensivity and precision for the serum fT₃ determination in cow.