Comparative Research on EPS Extraction from Mechanical Dewatered Sludge with Different Methods

摘要

In order to find a suitable extracellular polymer substance (EPS) extraction method for mechanical dewatered sludge, four different methods including EDTA extraction, alkali extraction, acid extraction, ultrasonic extraction method have been used in extracting EPS from belt filter dewatered sludge. The contents of polysaccharide and proteins extracted from the dewatered sludge by different extraction methods are also analyzed. The results indicated that EDTA method and alkali extraction method are more suitable for dewatered sludge with more EPS content and less cell damage, while sulfuric acid extraction and ultrasonic extraction were poorer with obvious cell lysis shown by higher DNA content in extracted EPS. Contents of proteins and polysaccharide in EPS extracted from mechanical dewatered sludge, is at the contents between that in EPS extracted from activated sludge and anaerobic digestion sludge.