Automated Multichamber Time-lapse Videography for Long-term In Vivo Observation of Migrating Cells

摘要

Aim: To observe and document the migration of living cells by time-lapse videography, we constructed a low-budget system based on a common inverted microscope. Materials and Methods: Long-term observation of six-well plates is enabled through maintenance of cell culture conditions (5% CO2 in air at 37{degrees}C). Points of interest can be revisited in definable intervals with <1 m repositioning error. Digital photographs from each programmed time point are paired with environmental data and combined into a record. Results: We used this new chamber to observe the migration of various cell lines. The design represents a good compromise between low cost and good precision. Detailed analyses verified that the environmental conditions were appropriately maintained, enabling long-term observation of viable cells. The stimulating influence of irradiation with photons (radiotherapy) on cellular motility of glioblastoma cells is presented. Conclusion: This study demonstrates that useful videographic systems can be constructed at low cost.