Seed Germination and in vitro Propagation of Agave grijalvensis an Endemic Endangered Mexican Species

摘要

In this study, seed germination and micropropagation protocols for Agave grijalvensis were optimized. A 24% in vitro seed germination occurred after surface sterilization of capsules in three steps in 70% ethanol, 0.1% HgCl₂ and 3.0% calcium hypochlorite solution and culturing on solid nutrient medium without any growth regulators. Maximum shoot induction was obtained with 38.2 μM 6-benzyl adenine (BA) and 0 μM 2,4-dichorophenoxyacetic acid (2,4-D). The concentration of 2,4-D increased the callus induction whereas BA and the interaction among BA and 2,4-D had no significant effect. Root induction was best on a ? -MS medium containing 22.7 μM indole butyric acid (IBA) and a 16/8 light regime. This protocol was efficient for obtained vigorous plants for transferred to greenhouse and further planted in field conditions.