Here, we present the first detailed histocompatibility study for flexible biocompatible implants and demonstrate the dras- tic differences in seizure onset between two types of electrodes: standard highly invasive platinum stimulators or our PEDOT:PSS biocompatible stimulators. We investigate the outcomes of a sub- thalamic nucleus (STN) implantation from a microscopic level with transmission electron microscopy (TEM) and fluorescence microscopy to a mesoscopic scale with after-discharge thresh- old modulation. Surrounding-implantation-site mitochondrias has been investigated through several parameters such as size, number or circularity for both the inorganic rigid and organic flexible elec- trode. Then fluorescence microscopy allowed us to demonstrate that surrounding brain cells health is dependent of the type of implant we used. Focusing on neurons, astrocytes, microglia and oligodendrocytes we showed that the glial scare is way less present or event not present at all around our flexible biocompatible stim- ulator. In addition to these histocompatibility results we wanted to know whether this highly significant differences in surround- ing probe environment can change macroscopic outcomes or not. To do that we manage to implant both a platinum stimulator in the hippocampus and one of our two types of probes in the STN, going through the dentate gyrus (DG). As predicted and according to our histological results, the after-discharge threshold is lower for platinum stimulators where it is not for the PEDOT:PSS ones.
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