Testing of Cotton Leaf Curl Virus Resistance of Candidate Varieties/strains Through Different Techniques

摘要

Twenty-seven upland cotton ( Gossypium hirsutum L.) cultivars were screened for their respond to cotton leaf curl virus (CLCV) by artificial inoculation through grafting in controlled condition. The plants of each cultivars/ strains grown in sterilized soil, and were graft inoculated with CLCV infected leaf of S-12 (petiole grafting techniques). Symptoms development was recorded till 60 days after grafting. Presence of CLCV was determined, 60 days after grafting through polymerase chain reaction (PCR). Nine strains/ cultivars demonstrated the typical symptoms after 13-38 days of grafting. One group (41/99, DNH-49, CP-15/2xCRIS-404, AENB-18/10/87, AENB-36/SE/90, BH-125 and SLH-242) showed severe symptoms (13-25 days after grafting), while the other group (MNH-633 and VH-140) developed mild symptoms (35-38 days after grafting). The third group (MNH-536, FH-900, FH-901, NIAB-98, NIAB-94, NIAB-801/F, RH-500, 13/99, CIM-473, CIM-482, VH-141, VH-142, VH-143, LRA-5166xCRIS-404, BH-121 and BH-124) remained asymptomatic. Polymerase chain reaction (PCR) of all the material was also done. The first two groups showed the amplification of virus DNA, while the third group was negative. We concluded that the cultivars /strains belonging to third group are highly resistance to CLCV infection in the Multan region.