Existence of bioactive flavonoids in rhizomes and plant cell cultures of Boesenbergia rotunda (L.) Mansf. Kulturpfl.

摘要

Callus and cell suspension cultures were explored as alternative sources of selected flavonoids and compared to those produced from rhizome of Boesenbergia rotunda (L.) Mansf. Kulturpfl. B. rotunda or fingerroot ginger is a medicinally important Zingiberaceae species. HPLC analysis showed the presence of alpinetin, pinocembrin, cardamonin, pinostrobin and panduratin A in the rhizomes, callus and cell suspension cultures of B. rotunda. Among the flavonoids, pinostrobin was predominant compared to panduratin A from the three sources. The rhizome extract of B. rotunda produced the highest quantity: a total of 5 selected flavonoids at 12975.52 ± 71.78 μg g-1 dry weight (DW). In contrast, callus and cells suspension extracts yielded 120.61 ? 0.01 and 3.14 ? 0.12 μg g-1 DW of the total selected flavonoids, respectively. However, this is the first report on the production of alpinetin, pinocembrin, cardamonin, pinostrobin and panduratin A from in vitro cultures of B. rotunda (L.) Mansf. Kulturpfl. Although the amount of flavonoids from invitro cultures was not comparable to the rhizome, it is crucial to initially establish invitro cultures and to proof the existence of these compounds in the cultures. This is a pre-requisite to enhance the accumulation of targeted bioactive compound through either metabolic engineering or chemical elicitation in future studies.