EVALUATION OF PROTECTIVE EFFECT OF BASSIA MALABARICA LEAVES AGAINST CISPLATININDUCED NEPHROTOXICITY AND DOXORUBICIN-INDUCED CARDIOTOXICITY IN RATS

摘要

Objective: The objective of the current investigation is to study the effect of ethanolic extract of Bassia malabarica leaves (EBML) against cisplatin-induced nephrotoxicity and doxorubicin (DOX)-induced cardiotoxicity in healthy adult male Wistar rats. Methods: Nephrotoxicity was induced by cisplatin (7 mg/kg, i.p ) and cardiotoxicity was induced by DOX (15 mg/kg, i.p ). In both the models, EBML (150 mg/kg and 300 mg/kg, p.o ) was administered for 15 days to assess the prophylactic and curative effect. Urinary parameters, biochemical parameters, and in vivo antioxidants were monitored for nephroprotective effect. For assessing cardioprotective effect serum parameters, cardiac ATPases and in vivo antioxidants were measured. A statistical significance was set at p<0.05 which was analyzed by one-way analysis of variance followed by Tukey’s multiple comparison test. Results: The study results show that the EBML has significantly (p<0.05) restored the urinary parameters, serum parameters of cisplatin-induced nephrotoxic rats, and serum parameters of DOX -induced cardiotoxic rats. A significant decrease (p<0.05) in levels of malondialdehyde and increase in reduced glutathione and catalase were seen in both nephrotoxic and cardiotoxic rats. Ca+2 ATPase was significantly decreased and Na+ K+ ATPase was significantly increased (p<0.05) in the treatment groups when compared to DOX disease control group. Conclusion: EBML showed a protective effect against cisplatin-induced nephrotoxicity and DOX -induced cardiotoxicity.