IDENTIFICATION OF PROTEINS ISOLATE FROM AMARANTH (AMARANTHUS CAUDATUS) BY SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS WITH WATER AND NACL 0.1 M SOLVENTS

摘要

Objective: The aim of this study was to obtain protein isolate from amaranth using alkaline method at extraction pH 8 and extraction pH 12 with different precipitation pHs and to analyze protein isolate with electrophoresis. Methods: Amaranth protein isolates were obtained using isoelectric precipitation method at different pHs. Proteins were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Results: A yield of 20.52% of protein isolate of defatted amaranth at pH 4.0 was obtained. The content of protein isolate was higher than 53% in all pH assays. Globulins and albumins in protein isolate at different pHs were observed. A band with 50 kDa corresponding to 7S globulin was found. The bands 36-38 kDa and 18-20 kDa correspond to 11S globulin. Bands less to 14.4 kDa correspond to albumins. Conclusions: Amaranth protein isolate is possible to obtain in extreme conditions of pH. The treatment with water was optimum to obtain amaranth protein isolate.