SPECTROPHOTOMETRIC DETERMINATION OF GLIPIZIDE IN BULK AND TABLET DOSAGE FORM BY ABSORPTION MAXIMA, FIRST ORDER DERIVATIVE SPECTROSCOPY AND AREA UNDER THE CURVE

摘要

Glipizide (GZ) is chemically 1cyclohexyl3[[p[2(5methylpyrazinecarboxamido)ethyl]phenyl] sulfonyl]urea, used in the treatment of type II diabetes mellitus. The drug is commercially available as tablets for oral administration. In the present work three simple, economical, precise and accurate UV spectrophotometric methods have been developed for the estimation of glipizide in bulk and pharmaceutical formulation. Method A is absorption maxima method in which λmaxwas found to be 274 nm. Method B is first order derivative spectroscopy where drug showed λmaxima=286 nm and λminima=263nm. Amplitude difference (dA/dλ) was calculated and was plotted against concentration and regression equation was calculated. Method C is area under the curve (AUC) in which area in the wavelength range of 255 nm - 295 nm was selected for analysis of glipizide. Linearity was observed in the concentration range 5-25 μg/ml ( r2 =0.999) for all the three methods. The % assay for the marketed formulation for absorption maxima, first order derivative and area under the curve method was found to be 99.03%, 100.16% and 99.06% respectively. The methods were validated with respect to linearity, precision and accuracy studies. Recovery studies for absorption maxima, first order derivative and area under the curve was found to be 100.83 %, 99.41% and 100.51% respectively. The methods were found to be simple, precise and accurate and can be employed for routine quality control analysis of glipizide in bulk as well as from its dosage form