Anticancer Effect of Cisplatin-Loaded Poly (Butylcyanoacrylate) Nanoparticles on A172 Brain Cancer Cells Line

摘要

Background: Drug delivery systems have been designed to achieve targeted delivery and control the release rateof the drugs. A serious challenge associated with drug delivery systems is the presence of the blood-brain barrier whichlimits drugs penetration. In the current study, the effects of cisplatin nanoparticles on A172 brain cancer cell line wereinvestigated. Methods: Cisplatin nanoparticles were produced by miniemulsion polymerization technique and theirproperties were evaluated. Drug release assay was performed to characterize the nanoparticles’ properties. Here, weexamined the effects of cisplatin nanoparticles and free form of cisplatin on A172 cancer cell line. MTT assay wasperformed for different concentrations of the drug. To measure the apoptosis rate in A172 cell line in the presenceof cisplatin nanoparticles or its free from, Annexin V staining method was used. Results: Our results indicated thatloading type of cisplatin was physical loading and only 4.7% of cisplatin was released after 68 h. Furthermore, MTTassay showed that cisplatin nanoparticles in all concentrations had more cytotoxic effects on the cells comparing withthe free form of cisplatin and control groups. We also showed that cisplatin nanoparticles could increase apoptosisin cancer cells more than the drug in the free form by using flow cytometry technique. Conclusion: Overall, thesefindings proved that cisplatin loaded on poly (Butylcyanoacrylate) nanoparticles, was more efficient than the free formof cisplatin in treating A172 cancer cell line.