ASSESSMENT OF PHYTOCHEMICAL COMPOSITION AND ANTIBACTERIAL ACTIVITY OF DIFFERENT EXTRACTS OF Barleria prionitis LEAVES AGAINST ORAL MICROFLORA TO IMPROVE DENTAL HYGIENE

摘要

Oral diseases are major health problem with dental caries. Oral health influence the general quality of life and poor oral health is linked to chronic conditions and systemic diseases. Hence there is an urgent need to find out an alternative way, instead of antibiotic and chemicals for the treatment of oral diseases which show more side effects. Present investigation is focused on antibacterial activity of aqueous, petroleum ether, chloroform and acetone extract of leaves of Barleria prionitis (Family-Acanthaceae) against seven bacterial species (Lactobacillus rhamnosus, Staphylococcus aureus , Bacillus subtilis , Actinomyces viscoscus, Staphylococcus epidermidis, Escherichia coli , Streptococcus mutans ) which are known to cause oral diseases in human being. Similarly leaves of Barleria prionitis were evaluated for its phytochemical composition. Antibacterial activity of the extracts was determined by paper disc diffusion method using 200mg/ml concentration of extract and there % zone of diffusion was calculated. Result showed that almost all the test organism from the present study were found to be effected by all the four type of extract of Barleria prionitis leaves but pronounced inhibition of the four extract was observed for bacterial species, Lactobacillus rhamnosus.Chloroform extract was found to be more effective against the entire test microorganism