Cellular Biocompatibility and Biomechanical Properties of N-carboxyethylchitosananohydroxyapatite Composites for Tissue-engineered Trachea

摘要

To prepare an NCECSHA composite for tissue-engineered trachea and investigate its biomechanical and biocompatibile properties. Biomechanical tests were performed on dry and wet NCECSHA composite specimens prepared in vitro. The cell adhesion rate on each composite surface after 2, 6, and 12 hours of culture was calculated, and cell proliferation activity was measured using an MTT assay. NCECSHA composites exhibited satisfactory tensile strength and Young's modulus values. The adhesion rate of rabbit tracheal chondrocytes on NCECSHA surfaces reached 88.4% after 12 hours of culture. NCECSHA composites are promising scaffold materials for tissue-engineered trachea owing to satisfactory biocompatible and biomechanical properties.