Osteogenesis Study of Hybrids of Indonesia?s Native Chicken Pelung ( Gallus gallus domesticus ) with Broiler ( Gallus gallus domesticus )

摘要

Objective: To examine bone development and its growth of hybrid of Indonesian native chick-pelung-( Gallus gallus domesticus ) and broiler ( Gallus gallus domesticus ) which was focused on anatomical and morphological properties of hybrid chick embryo?s skeleton. Methods: Eggs were incubated for a different period, which were 5, 10, 14, 18 and 22 days and 7 weeks, respectively. Whole mounts embryo staining with alcian blue-alizarin red was conducted for the bone development and ossification study. Bone histological examination of ossification mechanism was prepared by paraffin method. The examinations were conducted by macroscopic and microscopic observation. Results: The result showed that ossification of Hybrid embryo was started at around incubation days 10 at the cranium, which then followed by extremity and were completed after 18 days of incubation at the vertebrae and long bone. Intramembranous ossification was accomplished more advanced compared to endochondral ossification. Conclusion: The ossification on hybrid chicken were started at 10 days stage and accomplished in the period of 18-22 days stage of embryo development, which following two osteogenesis mechanisms; intramembranous and endochondral bone formation.