Prevention of ischemia-reperfusion lung injury during static cold preservation by supplementation of standard preservation solution with HEMO transplantation model

摘要

We describe the results of adding a new biological agent HEMO2life? to a standard preservation solution for hypothermic static lung preservation aiming to improve early functional parameters after lung transplantation. HEMO2life? is a natural oxygen carrier extracted from Arenicola marina with high oxygen affinity developed as an additive to standard organ preservation solutions. Standard preservation solution (Perfadex?) was compared with Perfadex? associated with HEMO2life? and with sham animals after 24?h of hypothermic preservation followed by lung transplantation. During five hours of lung reperfusion, functional parameters and biomarkers expression in serum and in bronchoalveolar lavage fluid (BALF) were measured. After five hours of reperfusion, HEMO2life? group led to significant improvement in functional parameters: reduction of graft vascular resistance (p?p?2life? group: expression of HMG B1 in serum tended to be lower in comparison (2.1?±?0.8 vs. 4.6?±?1.5) with Perfadex? group, TNF-α and IL-8 in BALF were significantly higher in the two experimental groups compared to control (p?? solution by an innovative oxygen carrier HEMO2life? during hypothermic static preservation improves early graft function after prolonged cold ischemia in lung transplantation.