ISOLATION AND IDENTIFICATION OF INFECTIOUS BRONCHITIS VIRUS FROM COMMERICIAL CHICKENS

摘要

Five infectious bronchitis viruses (IBV) were isolated from commercial chickens. One of them was isolated from a layer flock vaccinated with H120 on day old and the others were originated from broiler flocks without receiving the vaccine. All the isolates caused embryo mortality and or dwarfing, stunting, curling, clubbing of the down and urate deposits in the kindney. In tracheal orgn cultures (TOCs), the isolates caused typical ciliostasis. Electron microscopy confirmed all the isolates have coronavirus morphology. The isolates were also confirmed by dot-immunobinding assay using polyclonal antibody to IBV. In one-day-old specific pathogen free chicks, all the isolates could induce typical respiratory distress of IB at 36-48 h postinoculation by intraocular route. Virus-neutralization test showed that two isolates were recovered from broiler flocks did not neutralize by monospecific. Massachusetts (Mass) antiserum, indicating the presence of other serotypes in commercial chicken flocks in Iran .