Fourteen Polymorphic Microsatellite Markers for a Widespread Limestone Endemic, Carex eburnea (Cyperaceae: Carex sect. Albae)

摘要

Premise of the study : Microsatellite primers were developed for a widespread limestone endemic sedge, Carex eburnea , to facilitate investigation of the genetic diversity and phylogeography of this taxon and its closest relative, C. mckittrickensis . Methods and Results : Forty-eight primer pairs were designed from Illumina sequence data and screened for suitability. Fourteen of these primer pairs were polymorphic and generated one to seven alleles per locus. Cross-species amplifications were conducted for all four members of Carex sect. Albae . Conclusions : These primer pairs can be used to assess the genetic diversity and population structure in future studies of C. eburnea and C. mckittrickensis , and likely in other members of Carex sect. Albae .