A Solid-state Electrochemiluminescence Sensor for Detecting Glutathione with a Graphite-phase Carbon Nitride/Silica Modified Glassy Carbon Electrode

摘要

A solid-state electrochemiluminescence (ECL) sensor for the detection of reduced glutathione (GSH) based on a g-C_(3)N_(4)/SiO_(2) modified glass carbon electrode (GCE) has been developed in this research. The g-C_(3)N_(4), which is employed as a luminophore, is simply prepared and exhibits an excellent ECL response. Mesoporous silica hollow spheres (SiO_(2)) with a large specific surface area are introduced here to increase the loading amount of g-C_(3)N_(4). Compared to a g-C_(3)N_(4) modified GCE, the g-C_(3)N_(4)/SiO_(2) modified GCE displays a much higher ECL intensity. A high enhancement effect on the ECL intensity of g-C_(3)N_(4)/SiO_(2) modified GCE is obtained in the presence of GSH in the electrolyte. Moreover, the enhanced ECL intensity shows a good linear relationship to the GSH concentration in the range from 1.0 × 10~(?7) to 5.0 × 10~(?4) M, with a detection limit of 2.0 × 10~(?8) M (6.1 ng/mL). Besides, the ECL sensor exhibits a good anti-interference ability and has been successfully applied in the detection of GSH in commercial samples. The proposed sensor provides a promising platform for life science.