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Effects of Hypoxia Stress and Different Level of Mn2+ on Antioxidant Enzyme of Tomato Seedlings

机译:低氧胁迫和不同浓度的Mn2 +对番茄幼苗抗氧化酶的影响

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The changes of antioxidant enzyme activities and related genes expression of tomato seedlings were evaluated under hypoxia stress with different levels of Mn2+. Activities of superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxide (APX), glutathione reductase (GR), catalase (CAT), the contents of H2O2, ascorbic (AsA) and malondialdehyde (MDA) were studied to investigate how active oxygen damaged the membrane lipid under hypoxia stress. With 10-200 μmol?L-1 Mn2+, the activities of SOD, POD, APX, GR and the contents of H2O2, AsA, MDA of leaves and roots increased significantly, which indicated that low Mn2+ could eliminate the active oxygen and protect the membrane lipid from hurt. But the activities of catalase (CAT) decreased evidently in the root. When the concentration of Mn2+ reached 400-600 μmol?L-1 under hypoxia stress, the activities of SOD, POD, APX, GR and ASA content decreased remarkably. However, the contents of H2O2 and MDA increased contrarily. A series of resistance genes level achieved peak value with 10 μmol?L-1 Mn2+. The expression level of SOD, CAT, APX, POD, GR were 6.28, 2.19, 5.66, 5.21 and 6.79 times compared to control respectively. These results illustrated appropriate amount of Mn2+ could reduce the damage of active oxygen under hypoxia stress, but reversely, high level of Mn2+ just aggravated the already serious damage to the tomato seedlings.
机译:在缺氧胁迫下,不同浓度的Mn2 +对番茄幼苗抗氧化酶活性及相关基因表达的影响进行了评价。研究了超氧化物歧化酶(SOD),过氧化物酶(POD),抗坏血酸过氧化物(APX),谷胱甘肽还原酶(GR),过氧化氢酶(CAT),过氧化氢,抗坏血酸(AsA)和丙二醛(MDA)的活性,以研究其活性缺氧胁迫下氧气破坏了膜脂。在10-200μmol?L-1 Mn2 +下,叶片和根系的SOD,POD,APX,GR活性以及H2O2,AsA,MDA含量显着增加,这表明低Mn2 +可以消除活性氧并保护根系。膜脂不受伤害。但是过氧化氢酶(CAT)的活性在根部明显降低。当缺氧胁迫下Mn2 +浓度达到400-600μmol?L-1时,SOD,POD,APX,GR和ASA的活性显着下降。但是,过氧化氢和丙二醛的含量却相反地增加。一系列的抗性基因水平达到了10μmol?L-1 Mn2 +的峰值。与对照相比,SOD,CAT,APX,POD,GR的表达水平分别为6.28、2.19、5.66、5.21和6.79倍。这些结果表明适量的Mn2 +可以减少缺氧胁迫下活性氧的破坏,但是相反,高含量的Mn2 +加剧了已经严重的对番茄幼苗的破坏。

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