Agrobacterium Mediated Transformation of Vigna mungo (L.) Hepper with Cry1Ac Gene for Insect Resistance

摘要

Transfer of foreign genes into higher plants mediated by Agrobacterium tumefaciens is a standard technique in plant genetic engineering. We have produced of morphologically normal and fertile transgenic plants from leaf explants inoculated with Agrobacterium tumefaciens strain LBA 4404 carrying binary vector pCAMBIA 1319Z, the latter of which contains Cry1Ac gene for making insect tolerant blackgram plant, a neomycin phosphotransferase (nptII) gene and a β-glucuronidase (GUS) gene (uidA) for histochemical assay of the transgene. The transformed green shoots, selected and rooted on medium containing kanamycin, tested positive for nptII and uidA genes by histochemical assay and PCR analysis. These plantlets were established in soil and grown to maturity to collect the seeds. Analysis of T0 plants showed the expression and integration of uidA into the plant genome. GUS activity in leaves, roots, flowers, anthers and pollen grains was detected by histochemical assay. For insect bioassay, the transgenic plants were toxic to bollworm larvae and lessened the damage caused by their feeding.