Phosphate and potassium solubilizing bacteria from weathered materials of denatured rock mountain, Ha Tien, Kien Giang province, Vietnam

摘要

Twenty-five strains were isolated on Aleksandrov medium from sample soils/weathered rocks of Ha Tien Mountain, Kien Giang, Vietnam. Their colonies were round or irregular, white to yellow and their shape was rod, motile. Seventeen of twenty-five bacterial strains were identified by PCR technique with specific primers fd1 and rP, they were capable of dissolving both phosphate and potassium and seven strains had high phosphate and potassium dissolution capacity (>10 mg l~(-1) P2O5 and >50 mg l~(-1) K_2O) effectively. These seven strains were chosen to sequence, DNA sequencing were compared with GenBank database of NCBI by BLAST N software. The results showed that DNV16 strain was similarity of 99% with EU977655.1 (Microbacterium hominis), TC1D strain was a 99% similarity with FN547417.1 (Flectobacillus sp.), CH9E strain was a 99% similarity of 99% with GQ861463.1 (Agrobacterium tumefasciens), TC1A strain was similarity of 99% with HM003210.1 (Bacillus cereus) and FJ976533.1 (Bacillus coagulans), CH7A strain was 99% similarity with EU048539.1 (Bacillus cereus) and GQ214131.1 (Bacillus subtilis) and TD6B strain was a 99% similarity wih AB301013.1 (Bacillus subtilis) and CH7D strain was similarity of 98% with FJ976616.1 (Bacillus megaterium). Seven strains related with Bacillus megaterium and Bacillus coagulans closely in phylogenetic tree.