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Modification of Periodate Oxidation Method to Produce HRP-IgG Conjugate and Test its Stability Overtime

机译:修改高碘酸盐氧化方法以产生HRP-IgG缀合物并测试其稳定性

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Goat anti-Rabbit Horseradish Peroxidase (HRP) secondary conjugate was produced using a modified periodate oxidation method. The obtained conjugate was tested in the quality control techniques of therapeutic proteins. To determine the working dilution, titration of the prepared conjugate was performed in Indirect Enzyme-Linked Immunosorbent Assay (ELISA) and found to be 1:5000. This dilution was further tested in Western Blot analysis. The secondary conjugate was kept at 4 ° C for one month and its stability was verified by Western Blot and Indirect ELISA techniques.
机译:使用改良的高碘酸盐氧化方法生产山羊抗兔辣根过氧化物酶(HRP)二级缀合物。在治疗性蛋白质的质量控制技术中测试所获得的缀合物。为了确定工作稀释度,在间接酶联免疫吸附测定(ELISA)中进行了制备的结合物的滴定,发现为1:5000。在Western印迹分析中进一步测试了该稀释液。次级结合物保持在4℃。 C维持1个月,并通过Western Blot和间接ELISA技术验证了其稳定性。

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