Effect of extracellular products extract of cyanobacteria Oscillatoria tenuis on micropropagation of date palm Phoenix dactylifera L. cv. 'Barhee'.

摘要

The aim of the study was to determine the effect of the extract of extracellular products of cyanobacteria Oscillatoria tenuis as an alternative or complementary component of the MS medium prepared for the date palm Phoenix dactylifera shoot proliferation growing by in vitro culture. The results shows that the medium supplemented with a half-strength of MS salts and the extract of cyanobacteria extracellular products (CEP) gave significantly increase of the percentage response to shoot proliferation and rooting, number of shoot, primary and secondary root, fresh and dry weight of shoot and root, total chlorophyll content, N%, phosphorus and potassium content compared with other two treatments. But this treatment gave the lowest value in shoot length. Also the results indicate that the medium supplemented with the full strength of MS salts recorded the lowest values in the percentage response to shoot proliferation and shoot length, fresh and dry weight of shoot, total chlorophyll content, N% and phosphorus content. But the medium supplemented with the cyanobacteria extracellular products (CEP) extract gave the lowest value in number of shoots. While this treatment gave the highest value in length of the shoot compared to the other two treatments. While the medium supplied with the cyanobacteria extracellular products (CEP) extract gave the lowest value in leaf content of total. After the plants were obtained from the shoot proliferation by the micro propagation method of P. dactylifera cv. “Barhee” were acclimatized by a success rate of 65.2%.