Sodium metabisulphite induced polymerization of sickle cell haemoglobin incubated in extracts of three medicinal plants (Anacardium occidentale, Psidium guajava and Terminalia catappa)

摘要

The present?in vitro?study ascertained the capacity of three medicinal plants, namely,?Anacardium occidentale, Psidium guajava?and?Terminalia catappa, to alterpolymerization?of?sickle cell haemoglobin (HbS).?Spectrophotometric method was used to monitor the level of polymerization of haemolysate HbS molecules treated with sodium metabisulphite (Na2S2O5) at a regular interval of 30 s for a period of 180 s in the presence of separate aqueous extracts of?A. occidentale, P. guajavaand?T. catappa. At time intervals of 30 s, the level of polymerization was expressed as percentage of absorbance relative to?the?control sample at the 180th?s. Although, extracts of the three medicinal plants caused significant (p 60 s, extract concentration of 400 mg% of?A. occidentale?activated polymerization of deoxyHbS molecules by 6.23 ± 1.34, 14.53 ± 1.67, 21.15 ± 1.89 and 24.42 ± 1.09%, 800 mg% of?T. catappa?at t > 30 s?gave values of?2.50 ± 1.93, 5.09 ± 1.96, 10.00 ± 0.99, 15.38 ± 1.33 and 17.31 ± 0.97%. Therefore, the capacity of the three medicinal plants to interfere with polymerization of deoxyHbS molecules depended on duration of incubation and concentration of the extracts.