Effect of interaction of 6-benzyl aminopurine (BA) and sucrose for efficient microtuberization of two elite potato (Solanum tuberosum L.) cultivars, Desiree and Cardinal

摘要

Single node and multinode explants of two potato cvs., Desiree and Cardinal were tested for?in vitro?microtuber production. Explants were taken from tissue culture laboratory of Seed Centre, University of the Punjab, Lahore, Pakistan in 2004. Experiments were designed in completely randomized pattern.?34?media treatments of Murashige and Skoog (1962) with varying concentrations of sucrose (4, 6, 8, 10 and 12%) either alone or in combination (5-8%?sucrose) with 6-benzyl amino purine (BA) were studied.?In the case of cv. Desiree,?medium MB10?(BA 6 mg/l, sucrose 6%)?and for cv.?Cardinal, medium MK20 (BA 5 mg/l, sucrose 8%)?in term of induction, mean number and?mean fresh weight per single node?explant were optimized. In comparison, cv.?Desiree?genotypically was observed to be slightly slow in response to?in vitro?microtuber induction and development than cv. Cardinal.