Applied orthogonal design for filtrating conditions of ultrasonic-assisted extraction from plant-chicory

摘要

The objective of the current study is to achieve global optimization of ultrasound-assisted extraction from chicory roots using a mixed orthogonal array design. Eight conditional factors were examined in the mixed orthogonal array (L16?(43×26)). The results showed that the importance of the eight factors, in decreasing order, wasethanol content, impregnation repetitions, ultrasonic input power, sonication temperature, sonication repetitions, solvent-to-solid ratio, impregnation time and sonication time.?The optimum extraction conditions included a frequency of 40 kHz, an impregnation time of 24 h with two rounds of impregnation, a sonication period of 30 min and an ultrasonic input power of 400 W with two rounds of sonication.Importantly, these?conditions?were independent of?alcohol content,?solvent-to-solid ratio and sonication temperature.?At frequency of 40 kHz,?the?alcohol content,solvent-to-solid ratio and sonication temperature were optimized in the range of?50 to 75% (v/v),?32:1 and 50°C, respectively.